Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation
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چکیده
منابع مشابه
Synthesis of coumarin or ferrocene labeled nucleosides via Staudinger ligation
BACKGROUND Reaction of azides with triaryl phosphines under mild conditions gives iminophosphoranes which can react with almost any kind of electrophilic reagent, e.g. aldehydes/ketones to form imines or esters to form amides. This so-called Staudinger ligation has been employed in a wide range of applications as a general tool for bioconjugation including specific labeling of nucleic acids. ...
متن کاملDNA conjugation by Staudinger ligation.
Two 5 modified 2'-deoxyuridin triphosphates and a 7 modified 2'-deoxy-7-deazaadenosine were synthesized carrying a terminal azide linked to the base. For probing the sterical influence on incorporation and Staudinger ligation different sized flexible linkers were chosen. All three nucleotides can completely replace their natural counterparts in primer extension as well as polymerase chain react...
متن کاملProtein Assembly Using the Staudinger Ligation
Introduction New methods are facilitating the total chemical synthesis of proteins. In particular, the chemical ligation of synthetic peptides provides a convergent route to proteins. Currently, the most common ligation method is “native chemical ligation” [1]. In native chemical ligation, the thiolate of an N-terminal cysteine residue of one peptide attacks the C-terminal thioester of a second...
متن کاملBrillouin scattering from cross-linked gels
In this letter we report Brillouin scattering measurements on methyl-methacrylate (MMA) gels crosslinked with varying amounts of ethylene-dimethacrylate (EDMA). We find that the k dependence of the phase velocity changes on increasing the cross-link content. For higher concentrations of crosslink we observe maxima and minima in the k dependence of the phase velocity. We associate these minima a...
متن کاملStereoselective N-glycosylation by Staudinger ligation.
Stereoselective methods for the chemical synthesis of beta-N-glycosyl amides are needed to generate glycopeptides and glycoproteins. Here, we report that the Staudinger ligation can be used to form glycosylated asparagine derivatives. The reaction proceeds with high stereoselectivity, and a variety of glycosyl azides can function as substrates. Our results provide precedence for the use of this...
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ژورنال
عنوان ژورنال: Acta Biomaterialia
سال: 2011
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2010.07.016